Abstract
A new inositol derivative could be isolated from the Archaeum Pyrococcus woesei and identified as di-myo-inositol-1,1'-phosphate by 1H, 31P NMR spectroscopy, mass spectrometry and thin layer chromatography. In P. woesei, this inositol phosphate represents the dominant counterion of K+ which ranges from 500 to 600 mM. The role of the potassium salt of di-myo-inositol-1,1'-phosphate as thermostabilizer is discussed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Archaea / chemistry*
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Archaea / metabolism
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Chromatography, Thin Layer
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Enzyme Stability
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Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism
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Inositol Phosphates / isolation & purification*
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Inositol Phosphates / metabolism
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Potassium / metabolism
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Temperature
Substances
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Inositol Phosphates
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di-myo-inositol-1,1'-phosphate
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Glyceraldehyde-3-Phosphate Dehydrogenases
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Potassium