Abstract
To reduce the embryonic pathogenicity of Newcastle disease virus (NDV), escape mutants of the La Sota strain were produced with selected monoclonal antibodies. Immunoselection resulted in the elimination of an epitope by single amino acid substitution (F and HN molecule) or in a conformational change (HN molecule). The embryonic pathogenicity of these escape mutants was reduced and their dose was optimised for in ovo vaccination. Because antibody responses and protection of in ovo vaccinated chicks were similar to controls vaccinated at hatch with the La Sota strain, immunoselection appears a valuable technique to produce attenuated NDV strains, which are candidate in ovo vaccines.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / blood*
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Chick Embryo / immunology*
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Chick Embryo / virology
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Chickens / immunology*
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Epitopes / genetics
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Epitopes / immunology
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Genes, Viral
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HN Protein / analysis
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HN Protein / genetics
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HN Protein / immunology
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Hemagglutination Inhibition Tests
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Immunoglobulin G / blood
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Immunoglobulin M / blood
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Molecular Sequence Data
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Mutation*
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Newcastle Disease / prevention & control*
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Newcastle disease virus / genetics
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Newcastle disease virus / immunology*
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Newcastle disease virus / pathogenicity
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Sequence Analysis, DNA
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Specific Pathogen-Free Organisms
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Vaccines, Attenuated / administration & dosage
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Vaccines, Attenuated / immunology
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Vaccines, Attenuated / isolation & purification
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Viral Fusion Proteins / genetics
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Viral Fusion Proteins / immunology
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Viral Vaccines / administration & dosage
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Viral Vaccines / immunology*
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Viral Vaccines / isolation & purification
Substances
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Antibodies, Viral
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Epitopes
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HN Protein
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Immunoglobulin G
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Immunoglobulin M
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Vaccines, Attenuated
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Viral Fusion Proteins
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Viral Vaccines
Associated data
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GENBANK/AJ621747
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GENBANK/AJ629059
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GENBANK/AJ629060
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GENBANK/AJ629061
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GENBANK/AJ629062
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GENBANK/AJ629063
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GENBANK/AJ629064
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GENBANK/AJ629065
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GENBANK/AJ629066