Quantitative spectroscopy of superficial turbid media

Opt Lett. 2005 Dec 1;30(23):3165-7. doi: 10.1364/ol.30.003165.

Abstract

We report a novel diffuse optical spectroscopy probe design for determining optical properties of superficial volumes of turbid samples. The fiber-based probe employs a highly scattering layer placed in contact with the sample of interest. This layer diffuses photons from a collimated light source before they enter the sample and provides a basis for describing light transported in superficial media by the diffusion approximation. We compare the performance of this modified two-layer diffusion model with Monte Carlo simulations. A set of experiments that demonstrate the feasibility of this method in turbid tissue phantoms is also presented. Optical properties deduced by this approach are in good agreement with those derived by use of a benchmark method for determining optical properties. The average interrogation depth of the probe design investigated here is estimated to be less than 1 mm.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Equipment Design
  • Equipment Failure Analysis
  • Fiber Optic Technology / instrumentation*
  • Image Enhancement / instrumentation*
  • Image Enhancement / methods
  • Nephelometry and Turbidimetry / instrumentation*
  • Nephelometry and Turbidimetry / methods
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrum Analysis / instrumentation*
  • Spectrum Analysis / methods
  • Transducers*