Abstract
Alveolar cells were evaluated in BALB/c mice exposed to smoke from 9 cigarettes per day. The mice were sacrificed at 1, 5, and 10 days and examined by lung morphometry and immunohistochemical staining of alveolar macrophages for tumor necrosis factor (TNF)-alpha, matrix metalloproteinase (MMP)-12, and tissue inhibitor of metalloproteinase (TIMP)-2. Cigarette smoke (CS)-exposed mice showed a progressive increase in numbers of alveolar macrophages (AMs) up to 10 days. MMP-12 was increased in AMs compared to the control group. The number of AMs with TNF-alpha and TIMP-2 labeling was higher at 5 days but decreased at 10 days. Short-term CS exposure induced pulmonary inflammation with major participation of MMP-12-positive AMs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Count
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Immunohistochemistry
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Lung / cytology
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Lung / pathology
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Macrophages, Alveolar / chemistry*
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Macrophages, Alveolar / cytology
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Male
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Matrix Metalloproteinase 12
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Metalloendopeptidases / analysis*
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Metalloendopeptidases / metabolism
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Mice
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Mice, Inbred BALB C
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Pneumonia / metabolism
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Pneumonia / pathology
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Time Factors
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Tissue Inhibitor of Metalloproteinase-2 / analysis*
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Tissue Inhibitor of Metalloproteinase-2 / metabolism
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Tobacco Smoke Pollution*
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Tumor Necrosis Factor-alpha / analysis*
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Tobacco Smoke Pollution
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Tumor Necrosis Factor-alpha
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Tissue Inhibitor of Metalloproteinase-2
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Metalloendopeptidases
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Matrix Metalloproteinase 12