Abstract
Leishmania major parasites lacking the GDP-mannose transporter, termed Deltalpg2 parasites, fail to induce disease in mice but persist long-term. We previously found that Deltalpg2 organisms protect BALB/c mice from virulent L. major challenge. In contrast, we report here that Deltalpg2 parasites induce protective immunity in C57BL/6 mice only when administered with CpG-containing oligodeoxynucleotides, indicating that parasite persistence alone is not sufficient to maintain protective immunity to L. major.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Adjuvants, Immunologic / pharmacology*
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Animals
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Glycosphingolipids / deficiency*
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Glycosphingolipids / genetics*
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Glycosphingolipids / physiology
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Leishmania major / genetics
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Leishmania major / immunology*
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Leishmaniasis, Cutaneous / immunology*
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Leishmaniasis, Cutaneous / prevention & control
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Membrane Proteins / deficiency*
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Membrane Proteins / genetics*
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Membrane Proteins / physiology
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Protozoan Proteins / genetics*
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Protozoan Proteins / physiology
Substances
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Adjuvants, Immunologic
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Glycosphingolipids
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LPG2 protein, Leishmania
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Membrane Proteins
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Protozoan Proteins