Abstract
The acyl-homoserine lactone molecular species (AHLs) produced by the Yersinia pestis AHL synthase YspI were identified by biochemical and physical/chemical techniques. Bioassays of extracts from culture supernatants of the recombinant YspI and wild-type Yersinia pestis showed similar profiles of AHLs. Analysis by liquid chromatography-mass spectrometry revealed that the predominant AHLs were N-3-oxooctanoyl-L-homoserine lactone and N-3-oxo-hexanoyl-L-homoserine lactone.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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4-Butyrolactone / analogs & derivatives
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4-Butyrolactone / analysis
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4-Butyrolactone / metabolism
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Amino Acid Sequence
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Escherichia coli / metabolism
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Gas Chromatography-Mass Spectrometry
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Ligases / genetics
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Ligases / metabolism*
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Molecular Sequence Data
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Operon / genetics
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Recombinant Proteins / metabolism
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Yersinia pestis / enzymology
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Yersinia pestis / physiology*
Substances
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Recombinant Proteins
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homoserine lactone
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N-(3-oxohexanoyl)-3-aminodihydro-2(3H)-furanone
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Ligases
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N-acylhomoserine lactone synthase
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4-Butyrolactone