Abstract
A lignan derivative, (-)-(7R, 8S)-dihydrodehydrodiconiferyl alcohol (DHDA), was isolated from Kalopanax septemlobus L. and was observed to have neuritogenic activity. DHDA at 50 microM caused a marked induction of neurite outgrowth and an enhancement of nerve growth factor (NGF)-mediated neurite outgrowth from PC12 cells. However, it did not exhibit any neurotrophic action. At 50 microM, DHDA enhanced NGF-induced neurite-bearing activity. This activity was partially blocked by the mitogen-activated protein kinase (MAPK) inhibitor PD98059 and by GF109203X, a protein kinase C (PKC) inhibitor. These results suggest that DHDA can induce neurite outgrowth and enhance NGF-induced neurite outgrowth from PC12 cells by amplifying up-stream steps such as MAPK and PKC.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Araliaceae
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Dose-Response Relationship, Drug
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Enzyme Inhibitors / pharmacology
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Flavonoids / pharmacology
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Indoles / pharmacology
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Lignin / analogs & derivatives*
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Lignin / antagonists & inhibitors
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Lignin / isolation & purification
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Lignin / pharmacology
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MAP Kinase Signaling System / drug effects
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Maleimides / pharmacology
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Medicine, East Asian Traditional
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Mitogen-Activated Protein Kinase Kinases / antagonists & inhibitors
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Nerve Growth Factor / pharmacology
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Neurites / drug effects*
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Neurites / physiology
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PC12 Cells
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Plant Bark / chemistry
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Plant Stems
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Plants, Medicinal
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Protein Kinase C / antagonists & inhibitors
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Rats
Substances
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Enzyme Inhibitors
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Flavonoids
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Indoles
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Maleimides
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pinonesinol
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Lignin
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Nerve Growth Factor
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Protein Kinase C
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Mitogen-Activated Protein Kinase Kinases
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bisindolylmaleimide I
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2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one