A radiation-reduced hybrid cell line containing 5 Mb/17 cM of human DNA from 9q34

Genomics. 1992 Jul;13(3):841-4. doi: 10.1016/0888-7543(92)90165-o.

Abstract

Disease gene loci for tuberous sclerosis (TSC1), idiopathic torsion dystonia (DYT1), and nail-patella syndrome (NPS1) have been mapped by genetic linkage analysis to human chromosome 9q band 34. To create a resource for physical mapping and manipulation of this region of the genome, we have created a radiation-reduced hybrid cell line containing DNA from human 9q34 as its only human component. This cell line, E6B, has been characterized by Southern blot and PCR analysis using a panel of 9q markers and fluorescent in situ hybridization. We estimate that it contains 5 Mb of human DNA, equal to 17 cM of genetic distance, extending from AK1 to ABO on 9q34.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line
  • Chromosome Mapping / methods
  • Chromosomes, Human, Pair 9*
  • DNA / genetics*
  • Dystonia Musculorum Deformans / genetics
  • Fluorescence
  • Genetic Linkage
  • Humans
  • Hybrid Cells / radiation effects
  • Nail-Patella Syndrome / genetics
  • Nucleic Acid Hybridization
  • Tuberous Sclerosis / genetics

Substances

  • DNA