Heterologous expression of an Entamoeba histolytica chitin synthase in Saccharomyces cerevisiae

Eukaryot Cell. 2006 Jan;5(1):203-6. doi: 10.1128/EC.5.1.203-206.2006.

Abstract

Chitin in the cyst wall of Entamoeba histolytica is made by two chitin synthases (Chs), one of which is unique (EhCHS-1) and one of which resembles those of insects and nematodes (EhCHS-2). EhCHS-1 is deposited chitin in the lateral wall of transformed Saccharomyces cerevisiae Chs mutants, independent of accessory proteins (Chs4p to Chs7p) required by yeast Chs3p.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Catalysis
  • Chitin / metabolism
  • Chitin Synthase / genetics*
  • Chitin Synthase / metabolism*
  • Entamoeba histolytica / enzymology*
  • Entamoeba histolytica / genetics
  • Gene Expression
  • Mutation / genetics
  • Phylogeny
  • Saccharomyces cerevisiae / genetics*

Substances

  • Chitin
  • Chitin Synthase