Abstract
To increase the frequency of homologous recombination, we inactivated the KU80 homologue in Aspergillus fumigatus (named akuB(KU80)). Homologous integration reached about 80% for both calcineurin A (calA) and polyketide synthase pksP (alb1) genes in the akuB(KU80) mutant to 3 and 5%, respectively, when using a wild-type A. fumigatus strain. Deletion of akuB(KU80) had no influence on pathogenicity in a low-dose murine infection model.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Nuclear / genetics*
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Aspergillus fumigatus / cytology
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Aspergillus fumigatus / genetics*
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Aspergillus fumigatus / growth & development
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Aspergillus fumigatus / pathogenicity*
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Calcineurin / deficiency
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Calcineurin / genetics
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DNA-Binding Proteins / genetics*
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Genetic Techniques
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Genome, Fungal
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Ku Autoantigen
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Methyl Methanesulfonate / pharmacology
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Mice
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Mice, Inbred BALB C
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Mutation / genetics*
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Phenotype
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Recombination, Genetic / genetics*
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Virulence
Substances
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Antigens, Nuclear
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DNA-Binding Proteins
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Methyl Methanesulfonate
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Calcineurin
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Xrcc6 protein, mouse
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Ku Autoantigen