Gene targeting in Aspergillus fumigatus by homologous recombination is facilitated in a nonhomologous end- joining-deficient genetic background

Sven Krappmann; Christoph Sasse; Gerhard H Braus

doi:10.1128/EC.5.1.212-215.2006

Gene targeting in Aspergillus fumigatus by homologous recombination is facilitated in a nonhomologous end- joining-deficient genetic background

Eukaryot Cell. 2006 Jan;5(1):212-5. doi: 10.1128/EC.5.1.212-215.2006.

Authors

Sven Krappmann¹, Christoph Sasse, Gerhard H Braus

Affiliation

¹ Institute of Microbiology & Genetics, Department of Molecular Microbiology and Genetics, Grisebachstr. 8, D-37077 Göttingen, Germany. [email protected]

Abstract

The akuA gene encoding the Ku70 component of the nonhomologous end-joining machinery was deleted in the opportunistic pathogen Aspergillus fumigatus. No obvious phenotype could be assessed for the corresponding mutant strain but relative frequencies of homologous recombination were increased as deduced from targeting the laccase-encoding abr2 gene.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigens, Nuclear / genetics*
Aspergillus fumigatus / cytology
Aspergillus fumigatus / genetics*
Aspergillus fumigatus / pathogenicity
DNA-Binding Proteins / genetics*
Gene Deletion
Gene Targeting / methods*
Genes, Fungal / genetics
Ku Autoantigen
Recombination, Genetic / genetics*

Substances

Antigens, Nuclear
DNA-Binding Proteins
Ku Autoantigen