Abstract
To differentiate avian influenza virus (AIV)-infected chickens vs. chickens immunized with inactivated avian influenza virus, an enzyme-linked immunosorbent assay (ELISA) was developed using a recombinant nonstructural protein (NS1) as the diagnostic antigen, which was cloned from an AIV H9N2 subtype strain isolated during the avian influenza outbreak of 2003-04 and expressed in Escherichia coli. Antibodies to the AIV NS1 protein was only detected in the sera of chickens experimentally infected with AIV but not in the sera of chickens immunized with inactivated vaccine. This ELISA is useful for serological diagnosis to distinguish chickens infected with influenza viruses from those immunized with inactivated vaccine.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / blood*
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Base Sequence
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Chick Embryo
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Chickens
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Enzyme-Linked Immunosorbent Assay / methods
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Enzyme-Linked Immunosorbent Assay / statistics & numerical data
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Enzyme-Linked Immunosorbent Assay / veterinary*
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Genes, Viral
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Influenza A virus / genetics
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Influenza A virus / immunology*
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Influenza Vaccines / administration & dosage
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Influenza Vaccines / immunology
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Influenza in Birds / diagnosis*
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Influenza in Birds / immunology*
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RNA, Viral / genetics
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Reproducibility of Results
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Sensitivity and Specificity
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Vaccination / veterinary
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Vaccines, Inactivated / administration & dosage
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Vaccines, Inactivated / immunology
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Viral Nonstructural Proteins / genetics
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Viral Nonstructural Proteins / immunology*
Substances
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Antibodies, Viral
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INS1 protein, influenza virus
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Influenza Vaccines
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RNA, Viral
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Recombinant Proteins
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Vaccines, Inactivated
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Viral Nonstructural Proteins