Abstract
To increase the facility and throughput of scoring phenotypic traits in embryonic zebrafish, we developed an automated micro-well assay for heart rate using automated fluorescence microscopy of transgenic embryos expressing green fluorescent protein in myocardium. The assay measures heart rates efficiently and accurately over a large linear dynamic range, and it rapidly characterizes dose dependence and kinetics of small molecule-induced changes in heart rate. This is the first high-throughput micro-well assay for organ function in an intact vertebrate.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Biological Assay / methods*
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Cardiovascular Agents / toxicity*
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Dose-Response Relationship, Drug
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Drug Evaluation, Preclinical / methods
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Embryo, Nonmammalian / physiology
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Feasibility Studies
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Green Fluorescent Proteins / analysis*
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Heart / drug effects*
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Heart / embryology
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Heart / physiology*
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Heart Rate / drug effects*
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Heart Rate / physiology
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Myocardium / metabolism
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Time Factors
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Zebrafish
Substances
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Cardiovascular Agents
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Green Fluorescent Proteins
Associated data
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PubChem-Substance/4266412
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PubChem-Substance/4266413
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PubChem-Substance/4266415
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PubChem-Substance/4266416
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PubChem-Substance/4266417