Abstract
Hedgehog (Hh) signaling is an important regulator of embryonic patterning, tissue regeneration, stem cell renewal and cancer growth. A purine derivative named purmorphamine was previously found to activate the Hh pathway and affect osteoblast differentiation through an unknown mechanism. We demonstrate here that purmorphamine directly targets Smoothened, a critical component of the Hh signaling pathway.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Membrane / metabolism
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Cell Proliferation / drug effects*
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Cells, Cultured
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Dose-Response Relationship, Drug
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Hedgehog Proteins
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Mice
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Morpholines / pharmacology*
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NIH 3T3 Cells
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Osteoblasts / drug effects
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Osteoblasts / metabolism
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Purines / pharmacology*
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Receptors, G-Protein-Coupled / metabolism*
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Signal Transduction / drug effects*
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Signal Transduction / physiology
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Smoothened Receptor
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Stem Cells / drug effects
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Stem Cells / metabolism
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Tissue Engineering
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Trans-Activators / metabolism*
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Veratrum Alkaloids / pharmacology
Substances
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Hedgehog Proteins
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Morpholines
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Purines
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Receptors, G-Protein-Coupled
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Smo protein, mouse
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Smoothened Receptor
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Trans-Activators
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Veratrum Alkaloids
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purmorphamine
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cyclopamine
Associated data
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PubChem-Substance/7851482
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PubChem-Substance/7851483
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PubChem-Substance/7851484
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PubChem-Substance/7851485
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PubChem-Substance/7851486