Abstract
The interleukin-1 receptor/toll-like receptor (IL-1R/TLR) superfamily signaling involves myeloid differentiation factor 88 (MyD88) that acts as an important adapter protein. A Japanese flounder (Paralichthys olivaceus) MyD88 (jfMyD88) cDNA and gene were cloned, and found to have lengths of 1.5 and 3.01 kb, respectively. The ORF encodes 285 amino acids that contain a death domain and a Toll/IL-1 receptor domain. The gene is composed of 5 exons and 4 introns. The jfMyD88 gene is highly expressed in organs involved in immune functions, including the gills, intestines, kidney, skin and spleen. Three days after a fish was infected with Edwardsiella tarda, staining with anti-jfMyD88 polyclonal antibody revealed an increased population of MyD88-positive cells in the kidney and spleen. These results imply that MyD88 has an important role in the innate immune system in Japanese flounder.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / biosynthesis
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Adaptor Proteins, Signal Transducing / genetics
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Adaptor Proteins, Signal Transducing / immunology*
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Western / veterinary
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Edwardsiella tarda / immunology*
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Enterobacteriaceae Infections / immunology
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Enterobacteriaceae Infections / veterinary*
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Fish Diseases / immunology*
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Fish Diseases / microbiology
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Flounder / genetics*
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Flounder / immunology
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Immunohistochemistry / veterinary
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Kidney / immunology
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Kidney / microbiology
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Molecular Sequence Data
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Myeloid Differentiation Factor 88
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Reverse Transcriptase Polymerase Chain Reaction / veterinary
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Sequence Alignment
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Spleen / immunology
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Spleen / microbiology
Substances
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Adaptor Proteins, Signal Transducing
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Myeloid Differentiation Factor 88
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RNA, Messenger