Molecular bacterial diversity of a forest soil under residue management regimes in subtropical Australia

FEMS Microbiol Ecol. 2006 Jan;55(1):38-47. doi: 10.1111/j.1574-6941.2005.00006.x.

Abstract

A major operational change in exotic pine plantations of subtropical Australia has been the decision to retain postharvest residues on site. A long-term field experiment was established in February 1996 to examine the impacts of residue management regimes [i.e. the postharvest residues removed (G0R), natural amount of residues retained (G1R) and residue quantity doubled and retained (G2R)] on tree growth (F1 hybrid pine) and sustainable soil management. Twelve soil samples, which included the above three residue regimes with four replicates, were collected at plantation age 6.4 years. A 16S rRNA gene clone library was established following soil community DNA extraction, polymerase chain reaction amplification and cloning. A total of 324 clones, including 27 from each sample, were randomly selected and sequenced to represent the bacterial composition and diversity of the clone library and thus the soil bacterial community under the residue management regimes. Phylogenetic analyses indicated that Acidobacteria (37.6%) and Proteobacteria (35.6%) were the dominant components of the soil bacterial community, followed by Actinobacteria (14.7%), Chlamydiae/Verrucomicrobia (7.3%), Unclassified Bacteria (3.8%) and Gemmatimonadetes (1.0%). Analysis of molecular variance revealed that there was no significant difference in bacterial composition and diversity among the residue management regimes or their replicated samples.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinobacteria / classification
  • Actinobacteria / genetics
  • Actinobacteria / isolation & purification
  • Australia
  • Bacteria / classification
  • Bacteria / genetics
  • Bacteria / isolation & purification*
  • Chlamydiaceae / classification
  • Chlamydiaceae / genetics
  • Chlamydiaceae / isolation & purification
  • Ecosystem
  • Proteobacteria / genetics
  • Proteobacteria / isolation & purification
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Soil Microbiology*
  • Species Specificity

Substances

  • RNA, Bacterial
  • RNA, Ribosomal, 16S