Abstract
Some of the elements involved in the erythroid-specific transcriptional regulation of the human gamma- and beta-globin genes and located inside or in the immediate proximity of these genes have been identified as sequences which bind erythroid-specific factors. In the present study, we found two regions located within 1 kb in 5' to the alpha 2- and in 3' to the alpha 1-globin genes which contribute to the induction of human alpha-globin genes following erythroid differentiation in stable MEL transformants. By DNAse I footprinting and gel mobility shift assays, we identified several GATA-1 and one AP-1/NF-E2-binding sites located inside these regions. These results strengthen the idea that, like for all other globin genes, flanking regions contribute in vivo to the regulation of human alpha-globin gene expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Binding Sites
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DNA Fingerprinting
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DNA-Binding Proteins / analysis*
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DNA-Binding Proteins / metabolism
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Deoxyribonuclease I / genetics
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Erythroid-Specific DNA-Binding Factors
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GATA1 Transcription Factor
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Gene Expression
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Gene Expression Regulation, Leukemic
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Globins / genetics*
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Humans
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Leukemia, Erythroblastic, Acute / pathology
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Molecular Sequence Data
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NF-E2 Transcription Factor
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NF-E2 Transcription Factor, p45 Subunit
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Oligonucleotides / analysis
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Proto-Oncogene Proteins c-jun / metabolism*
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Regulatory Sequences, Nucleic Acid
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Transcription Factors / metabolism
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Tumor Cells, Cultured
Substances
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DNA-Binding Proteins
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Erythroid-Specific DNA-Binding Factors
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GATA1 Transcription Factor
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GATA1 protein, human
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NF-E2 Transcription Factor
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NF-E2 Transcription Factor, p45 Subunit
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NFE2 protein, human
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Oligonucleotides
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Proto-Oncogene Proteins c-jun
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Transcription Factors
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Globins
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Deoxyribonuclease I