Protective immunization against "Candidatus Helicobacter suis" with heterologous antigens of H. pylori and H. felis

Vaccine. 2006 Mar 24;24(14):2469-76. doi: 10.1016/j.vaccine.2005.12.033. Epub 2006 Jan 4.

Abstract

"Helicobacter (H.) heilmannii" type 1 colonizes the human stomach. It has been shown to be identical to "Candidatus H. suis", a Helicobacter species colonizing the stomach of more than 60% of slaughter pigs. This bacterium is, until now, not isolated in vitro. The effect of vaccination on "Candidatus H. suis" infection was studied in a mouse model. Mice were vaccinated intranasally or subcutaneously with whole bacterial cell lysate of Helicobacter pylori or Helicobacter felis and subsequently challenge infected with "Candidatus H. suis". Intranasal and subcutaneous immunisation caused a decrease in faecal excretion of "Candidatus H. suis" DNA. Urease tests on stomach tissue samples at 16 weeks after challenge infection were negative in all H. felis intranasally immunized animals and in the majority of the animals of the other immunisation groups. Since PCR on stomach tissue samples at 16 weeks after challenge infection could still detect "Candidatus H. suis DNA" in all immunisation-challenge groups, complete clearance of challenge bacteria was not achieved.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Intranasal
  • Animals
  • Antigens, Bacterial / administration & dosage
  • Antigens, Bacterial / immunology
  • Antigens, Heterophile / administration & dosage*
  • Antigens, Heterophile / immunology
  • Bacterial Vaccines / administration & dosage*
  • Bacterial Vaccines / immunology
  • DNA, Bacterial / analysis
  • Helicobacter Infections / prevention & control*
  • Helicobacter felis / chemistry*
  • Helicobacter heilmannii / drug effects*
  • Helicobacter heilmannii / metabolism
  • Helicobacter pylori / chemistry*
  • Immunization
  • Injections, Subcutaneous
  • Mice
  • Mice, Inbred BALB C
  • Polymerase Chain Reaction

Substances

  • Antigens, Bacterial
  • Antigens, Heterophile
  • Bacterial Vaccines
  • DNA, Bacterial