Abstract
Immune responses in the guinea pig model are understudied because of a lack of commercial reagents. We have developed a custom-made guinea pig oligonucleotide microarray (81 spots) and have examined the gene expression profile of splenocytes restimulated in vitro from Mycobacterium bovis BCG-vaccinated and naive animals. Eleven genes were significantly (P < 0.05) up-regulated following vaccination, indicating a Th1-type response. These results show that microarrays can be used to more fully define immune profiles of guinea pigs.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Outbred Strains
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BCG Vaccine / administration & dosage*
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BCG Vaccine / immunology*
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Cells, Cultured
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Chemokines / genetics
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Chemokines / metabolism
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Cytokines / genetics
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Cytokines / metabolism
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Disease Models, Animal
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Gene Expression Regulation
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Guinea Pigs
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Lymphocyte Activation
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Mycobacterium bovis / immunology*
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Oligonucleotide Array Sequence Analysis / methods*
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Spleen / cytology
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Spleen / immunology*
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Tuberculin / administration & dosage
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Tuberculin / immunology
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Tuberculosis / immunology*
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Tuberculosis / prevention & control
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Vaccination
Substances
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BCG Vaccine
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Chemokines
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Cytokines
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Tuberculin