[Antiapoptotic functions of the retrovirally transferred API2-MALT1 gene]

Rinsho Ketsueki. 2005 Jul;46(7):479-85.
[Article in Japanese]

Abstract

t(11 ; 18)(q21 ; q21) is a specific chromosomal aberration in mucosa-associated lymphoid tissue (MALT) lymphoma, and produces chimeric transcript Apoptosis Inhibitor 2 (API2)-MALT1. Although it is known that API2 has an antiapoptotic effect, it is still unclear whether this also applies to API2-MALT1. To investigate its effects against various apoptotic stimuli, API2-MALT1 was expressed by means of retroviral infection on the epithelial cell line HeLa and the murine Pro-B cell line Ba/F3. On both these cell lines, API2-MALT1 was found to cause a significant reduction in UV-induced apoptosis. The apoptosis induced by doxorubicin was also inhibited by API2-MALT1, but not that induced by IL-3 withdrawal from Ba/F3. These findings suggest that API2-MALT1 has an antiapoptotic effect on both epithelial and lymphoid cells and that this effect depends on the apoptotic stimulus.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Apoptosis / genetics*
  • Chromosomes, Human, Pair 11
  • Chromosomes, Human, Pair 18
  • Doxorubicin / pharmacology
  • Gene Expression Regulation, Developmental*
  • Gene Transfer Techniques*
  • Genetic Vectors / genetics*
  • HeLa Cells
  • Humans
  • Lymphoma, B-Cell, Marginal Zone / genetics*
  • Mice
  • Oncogene Proteins, Fusion / genetics*
  • Oncogene Proteins, Fusion / physiology*
  • Retroviridae / genetics*
  • Translocation, Genetic

Substances

  • API2-MALT1 fusion protein, human
  • Oncogene Proteins, Fusion
  • Doxorubicin