PNA-based reagents for the direct and site-specific synthesis of thymine dimer lesions in genomic DNA

J Carsten Pieck; David Kuch; Friederike Grolle; Uwe Linne; Clemens Haas; Thomas Carell

doi:10.1021/ja056358i

PNA-based reagents for the direct and site-specific synthesis of thymine dimer lesions in genomic DNA

J Am Chem Soc. 2006 Feb 8;128(5):1404-5. doi: 10.1021/ja056358i.

Authors

J Carsten Pieck¹, David Kuch, Friederike Grolle, Uwe Linne, Clemens Haas, Thomas Carell

Affiliation

¹ Department of Chemistry and Biochemistry, Ludwig-Maximilians University, Butenandtstr. 5-13, 83177 Munich, Germany.

PMID: 16448083
DOI: 10.1021/ja056358i

Abstract

An acetophenone containing PNA-based reagent was designed for the direct and site-specific synthesis of a cis-syn thymidine dimer lesion in genomic DNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetophenones / chemical synthesis
Acetophenones / chemistry*
Bacteriophages / genetics
Base Sequence
DNA / chemical synthesis*
DNA / chemistry
DNA / genetics
Deoxyribonucleases, Type II Site-Specific / chemistry
Horseradish Peroxidase / chemistry
Molecular Sequence Data
Peptide Nucleic Acids / chemistry*
Pyrimidine Dimers / chemical synthesis*
Streptavidin / chemistry
Substrate Specificity

Substances

Acetophenones
Peptide Nucleic Acids
Pyrimidine Dimers
DNA
Streptavidin
Horseradish Peroxidase
endodeoxyribonuclease PvuI
Deoxyribonucleases, Type II Site-Specific
acetophenone