The Raf inhibitor BAY 43-9006 (Sorafenib) induces caspase-independent apoptosis in melanoma cells

Cancer Res. 2006 Feb 1;66(3):1611-9. doi: 10.1158/0008-5472.CAN-05-0808.

Abstract

Mitogen-activated protein kinase (MAPK) is activated in the majority of melanomas, and its activity is essential for cell survival. In this report, we examined the effects of a novel raf inhibitor BAY 43-9006 on melanoma cell viability and intracellular signaling and found that it induces apoptosis through a caspase-independent mechanism. At concentrations that suppress extracellular signal-regulated kinase (ERK) phosphorylation, BAY 43-9006 dephosphorylates Bad on Ser(75) and Ser(99), activates Bak and Bax, and reduces the mitochondrial transmembrane potential. BAY 43-9006 (sorafenib) down-modulates the levels of Bcl-2 and Bcl-X(L) in a MAPK-independent manner in A2058 and SKMEL5 melanoma cells but not in the more resistant A375 cells. Of the three lines tested, only A375 cells were rescued from BAY 43-9006-induced apoptosis by knocking down Bad. BAY 43-9006 induced poly(ADP-ribose) polymerase cleavage and the mitochondrial release of cytochrome c and SMAC. However, the pan-caspase inhibitor Z-VAD-fmk had only a modest protective effect against the drug, suggesting that BAY 43-9006-induced apoptosis is largely caspase independent. BAY 43-9006 but not the MAP/ERK kinase inhibitors PD98059 or U0126 induced the nuclear translocation of apoptosis-inducing factor (AIF) in A2058 and SKMEL5 cells, and the introduction of a small interfering RNA (siRNA) for AIF partially protected these cells from BAY 43-9006-induced apoptosis. The AIF siRNA had little effect in A375 cells, in which drug-induced AIF release was negligible. These data indicate that in sensitive cell lines, BAY 43-9006-induced apoptosis is independent of Bad dephosphorylation and caspase activation and largely mediated through the nuclear translocation of AIF.

Publication types

  • Research Support, N.I.H., Extramural
  • Retracted Publication

MeSH terms

  • Apoptosis / drug effects*
  • Apoptosis Regulatory Proteins
  • Benzenesulfonates / pharmacology*
  • Caspases / metabolism
  • Cell Line, Tumor
  • Cell Nucleus / metabolism
  • Cytochromes c / metabolism
  • Humans
  • Intracellular Signaling Peptides and Proteins / metabolism
  • Melanoma / drug therapy*
  • Melanoma / enzymology
  • Melanoma / pathology*
  • Mitochondria / drug effects
  • Mitochondria / metabolism
  • Mitochondrial Proteins / metabolism
  • Mitogen-Activated Protein Kinases / antagonists & inhibitors
  • Niacinamide / analogs & derivatives
  • Phenylurea Compounds
  • Phosphorylation / drug effects
  • Proto-Oncogene Proteins c-bcl-2 / metabolism
  • Pyridines / pharmacology*
  • Sorafenib
  • bcl-Associated Death Protein / metabolism
  • raf Kinases / antagonists & inhibitors*

Substances

  • Apoptosis Regulatory Proteins
  • BAD protein, human
  • Benzenesulfonates
  • DIABLO protein, human
  • Intracellular Signaling Peptides and Proteins
  • Mitochondrial Proteins
  • Phenylurea Compounds
  • Proto-Oncogene Proteins c-bcl-2
  • Pyridines
  • bcl-Associated Death Protein
  • Niacinamide
  • Cytochromes c
  • Sorafenib
  • raf Kinases
  • Mitogen-Activated Protein Kinases
  • Caspases