Unique NS5b hepatitis C virus gene sequence consensus database is essential for standardization of genotype determinations in multicenter epidemiological studies

J Clin Microbiol. 2006 Feb;44(2):614-6. doi: 10.1128/JCM.44.2.614-616.2006.

Abstract

A multicenter study of NS5b hepatitis C virus (HCV) genotype determination involving 12 laboratories demonstrates that any laboratory with expertise in sequencing techniques would be able to provide a reliable HCV genotype for clinical and epidemiological purposes as long as they are provided a consensus reference sequence database.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Consensus Sequence
  • Databases, Genetic*
  • Genotype
  • Hepacivirus / classification*
  • Hepacivirus / genetics
  • Hepatitis C / epidemiology*
  • Hepatitis C / virology
  • Humans
  • Laboratories / standards*
  • Polymerase Chain Reaction
  • Reference Standards
  • Sequence Analysis, DNA
  • Viral Nonstructural Proteins / genetics*

Substances

  • Viral Nonstructural Proteins
  • NS-5 protein, hepatitis C virus