Objectives: The aim was to design a method for genotyping MMP-9 C-1562T gene polymorphism that does not require RFLP PCR or sequencing.
Design and methods: Optimization of tetra-primer ARMS PCR was performed to obtain the highest amount of specific amplification products.
Results: PCR products (436 bp, 296 bp, 220 bp) were visualized on MADGE or agarose gel electrophoresis.
Conclusions: We designed a faster, single-step, economical method which becomes advantageous for high throughput population screening.