Abstract
Methyl protodioscin (NSC-698790) is one of the main bioactive components in the traditional Chinese medicine Dioscorea collettii var. hypoglauca (Dioscoreaceae). In this study, we investigated the anti-proliferative effect of methyl protodioscin on the HepG2 cells and the mechanism of the induced cytotoxicity. Treatment of methyl protodioscin resulted in G2/M arrest and apoptosis in HepG2 cells. These effects were attributed to down-regulation of Cyclin B1 and the signaling pathways leading to up-regulation of Bax and down-regulation of BCL2, suggesting that methyl protodioscin may be a novel anti-mitotic agent.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Apoptosis / drug effects*
-
Blotting, Western
-
CDC2-CDC28 Kinases / metabolism
-
Cell Division / drug effects*
-
Cell Line, Tumor
-
Cyclin B / metabolism
-
Cyclin B1
-
Diosgenin / analogs & derivatives*
-
Diosgenin / pharmacology
-
G2 Phase / drug effects*
-
Humans
-
Liver Neoplasms / pathology*
-
Proto-Oncogene Proteins c-bcl-2 / metabolism
-
Saponins / pharmacology*
-
bcl-2-Associated X Protein / metabolism
Substances
-
CCNB1 protein, human
-
Cyclin B
-
Cyclin B1
-
Proto-Oncogene Proteins c-bcl-2
-
Saponins
-
bcl-2-Associated X Protein
-
methyl protodioscin
-
CDC2-CDC28 Kinases
-
Diosgenin