Multiplex PCR-RFLP methods for pfcrt, pfmdr1 and pfdhfr mutations in Plasmodium falciparum

Maria Isabel Veiga; Pedro Eduardo Ferreira; Anders Björkman; José Pedro Gil

doi:10.1016/j.mcp.2005.10.003

Multiplex PCR-RFLP methods for pfcrt, pfmdr1 and pfdhfr mutations in Plasmodium falciparum

Mol Cell Probes. 2006 Apr;20(2):100-4. doi: 10.1016/j.mcp.2005.10.003. Epub 2006 Feb 7.

Authors

Maria Isabel Veiga¹, Pedro Eduardo Ferreira, Anders Björkman, José Pedro Gil

Affiliation

¹ Center of Molecular and Structural Biomedicine, Universidade do Algarve, Gambelas, Portugal.

PMID: 16460912
DOI: 10.1016/j.mcp.2005.10.003

Abstract

Plasmodium falciparum drug resistance is a major factor for the death toll of malaria. Resistance has been associated with specific single nucleotide polymorphisms (SNPs) in the parasite genes pfmdr1 (N86Y) and pfcrt (K76T) associated with quionoline antimalarial resistance, and pfdhfr (N51I, C59R, S108N) correlated with resistance of the antifolate combination sulfadoxine-pyrimethamine. These SNPs constitute the basis for the surveillance of drug resistance through high sensitive molecular methods in malaria endemic countries. In this work, we developed a multiplex PCR-RFLP protocols for the diagnosis of these molecular markers, leading to significant decreases in reagent costs, time, number of manipulations and hence human resources.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics*
Animals
Drug Resistance / genetics
Membrane Proteins / genetics*
Membrane Transport Proteins
Mutation
Plasmodium falciparum / genetics*
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide
Protozoan Proteins / genetics*
Tetrahydrofolate Dehydrogenase / genetics*

Substances

ATP Binding Cassette Transporter, Subfamily B, Member 1
Membrane Proteins
Membrane Transport Proteins
PfCRT protein, Plasmodium falciparum
Protozoan Proteins
Tetrahydrofolate Dehydrogenase