Abstract
An indirect three step ELISA has been assessed in order to detect the possible release of cytochrome c, a mitochondrial protein, from isolated and perfused guinea-pig heart. The ELISA described in this study is sufficiently sensitive and accurate to measure extracellular cytochrome c.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cytochrome c Group / analysis*
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Cytochrome c Group / metabolism
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Cytochrome c Group / standards
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Enzyme-Linked Immunosorbent Assay / methods*
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Enzyme-Linked Immunosorbent Assay / standards
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Evaluation Studies as Topic
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Guinea Pigs
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Heart / physiology*
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In Vitro Techniques
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Male
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Perfusion
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Reference Standards
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Sensitivity and Specificity