Functional size measurements of the ubiquinol oxidase activity of the cytochrome o terminal oxidase complex of Escherichia coli

Biochem J. 1991 Jun 1;276 ( Pt 2)(Pt 2):555-7. doi: 10.1042/bj2760555.

Abstract

The functional molecular mass for ubiquinol-1 oxidation by the Escherichia coli terminal oxidase, cytochrome o, was determined by radiation-inactivation analysis of membranes from a cytochrome d-deficient mutant. The functional molecular mass for ubiquinol-l oxidase activity was found to be 38.3 +/- 2.65 kDa.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Membrane / enzymology
  • Electron Transport Complex IV / chemistry
  • Electron Transport Complex IV / metabolism*
  • Escherichia coli / enzymology*
  • Kinetics
  • Malate Dehydrogenase / metabolism
  • Malate Dehydrogenase / radiation effects
  • Molecular Weight
  • Quinone Reductases / chemistry
  • Quinone Reductases / metabolism*
  • Quinone Reductases / radiation effects

Substances

  • Malate Dehydrogenase
  • Quinone Reductases
  • ubiquinol-1 oxidase
  • cytochrome o oxidase
  • Electron Transport Complex IV