Abstract
A combined serological and PCR method for the detection of Listeria monocytogenes infection in symptomatic and asymptomatic ovine flocks was evaluated. Seventy-eight milk samples and 157 serum samples were analysed using a L. monocytogenes PCR detection kit and an anti-listeriolysin O IgG immunoassay kit. The combined use of these commercial kits allowed a rapid and effective detection of L. monocytogenes infection in both the early stage, before seroconversion, and in a later phase, even after antibiotic therapy.
MeSH terms
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Animals
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Antibodies, Bacterial / blood
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Bacterial Toxins / chemistry
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Enzyme-Linked Immunosorbent Assay / veterinary
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Female
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Heat-Shock Proteins / chemistry
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Hemolysin Proteins
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Histocytochemistry / veterinary
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Listeria monocytogenes / genetics
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Listeria monocytogenes / isolation & purification*
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Listeriosis / blood
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Listeriosis / microbiology
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Listeriosis / veterinary*
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Mass Screening / methods
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Mass Screening / veterinary
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Milk / microbiology
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Polymerase Chain Reaction / veterinary
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Sheep
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Sheep Diseases / blood
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Sheep Diseases / microbiology*
Substances
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Antibodies, Bacterial
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Bacterial Toxins
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DNA, Bacterial
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Heat-Shock Proteins
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Hemolysin Proteins
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hlyA protein, Listeria monocytogenes