Reduction by interferon-alpha of levels of c-myc protein and DNA synthesis in a human hepatoma cell line mediated by inhibition of putrescine synthesis

T Takeda; S Nishiguchi; T Kuroki; K Kobayashi; T Hasuma; I Matsui-Yuasa; S Otani

doi:10.1016/0006-291x(91)91824-v

Reduction by interferon-alpha of levels of c-myc protein and DNA synthesis in a human hepatoma cell line mediated by inhibition of putrescine synthesis

Biochem Biophys Res Commun. 1991 Jul 15;178(1):378-84. doi: 10.1016/0006-291x(91)91824-v.

Authors

T Takeda¹, S Nishiguchi, T Kuroki, K Kobayashi, T Hasuma, I Matsui-Yuasa, S Otani

Affiliation

¹ Third Department of Internal Medicine, Osaka City University Medical School, Japan.

PMID: 1648912
DOI: 10.1016/0006-291x(91)91824-v

Abstract

Interferon-alpha inhibited increases in ornithine decarboxylase, intracellular putrescine, and DNA synthesis as human hepatoma cells were stimulated to grow. Interferon-alpha inhibited the increase in the c-myc protein level, but not its mRNA level. Added putrescine abrogated the effects on c-myc protein and DNA synthesis. Interferon-alpha seemed to inhibit the increase in the c-myc protein level post-transcriptionally by reducing the putrescine level, inhibiting DNA synthesis.

MeSH terms

Blotting, Northern
Carcinoma, Hepatocellular
Cell Line
DNA Replication / drug effects*
Humans
Interferon Type I / pharmacology*
Kinetics
Liver Neoplasms
Ornithine Decarboxylase / metabolism*
Polyamines / metabolism*
Proto-Oncogene Proteins c-myc / biosynthesis*
Putrescine / antagonists & inhibitors
Putrescine / biosynthesis*
Putrescine / pharmacology
RNA, Messenger / genetics
RNA, Messenger / metabolism

Substances

Interferon Type I
Polyamines
Proto-Oncogene Proteins c-myc
RNA, Messenger
Ornithine Decarboxylase
Putrescine