Use of streptomycin for precipitation and detection of proteinase K resistant prion protein (PrPsc) in biological samples

Chem Commun (Camb). 2006 Mar 7:(9):973-5. doi: 10.1039/b516965h. Epub 2006 Jan 20.

Abstract

The ability of streptomycin to form multimolecular aggregates with pathogenic prion proteins and their recovery by precipitation via a low-speed centrifugation step has been demonstrated; these novel properties of streptomycin make it a useful substance that increases the sensitivity of laboratory diagnostic techniques for prion infections in man and animals.

MeSH terms

  • Animals
  • Cattle
  • Chemical Precipitation
  • Endopeptidase K / metabolism*
  • Molecular Structure
  • PrPSc Proteins / isolation & purification*
  • PrPSc Proteins / metabolism*
  • Solubility
  • Streptomycin / chemistry*

Substances

  • PrPSc Proteins
  • Endopeptidase K
  • Streptomycin