A Latex Agglutination Test (LAT) was developed for quick detection of hemagglutinin serum antibodies of H5 Avian Influenza Virus (AIV) in chicken. Recombinant hemagglutinin protein of H5 AIV were obtained and purified, then HA protein were covalently linked to carboxylated polyethylene latex beads by EDAC. The sensitisation condition such as ionic strength of the diluting mixture pH, concentration, antigen, the times of linking were optimized. 126 fresh serum after vaccination were detected by this LAT and HI simultaneously, the result show that sensitivity for the LAT was 87.03%, the specificity was 88.8%, the coincidence rate of both methods was 87.30%. The result indicated that this LAT method can be used for quick detection of serum antibodies and epidemiological study of H5 AIV.