Abstract
The protein content of melanosomes in the retinal pigment epithelium (RPE) was analyzed by mass spectrometry. More than 100 proteins were found to be common to two out of three variations of sample preparation. Some proteins normally associated with other organelles were detected. Several lysosomal enzymes were detected, with the presence of cathepsin D confirmed by immunoelectron microscopy, thus supporting the previously suggested notion that melanosomes may contribute to the degradation of ingested photoreceptor outer segment disks.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Cells, Cultured
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Epithelial Cells / chemistry
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Epithelial Cells / cytology
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Epithelial Cells / ultrastructure
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Mass Spectrometry
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Melanosomes / chemistry*
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Melanosomes / metabolism*
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Melanosomes / ultrastructure
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Microscopy, Immunoelectron
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Pigment Epithelium of Eye / chemistry*
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Pigment Epithelium of Eye / cytology
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Pigment Epithelium of Eye / ultrastructure
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Proteome / chemistry*
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Proteome / metabolism
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Retina / chemistry*
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Retina / cytology
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Retina / ultrastructure
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Swine