Expression level of vascular endothelial growth factor-C and -A in cultured human oral squamous cell carcinoma correlates respectively with lymphatic metastasis and angiogenesis when transplanted into nude mouse oral cavity

Takayuki Nakazato; Susumu Shingaki; Nobutaka Kitamura; Chikara Saito; Ryozo Kuwano; Masayoshi Tachibana

Expression level of vascular endothelial growth factor-C and -A in cultured human oral squamous cell carcinoma correlates respectively with lymphatic metastasis and angiogenesis when transplanted into nude mouse oral cavity

Oncol Rep. 2006 Apr;15(4):825-30.

Authors

Takayuki Nakazato¹, Susumu Shingaki, Nobutaka Kitamura, Chikara Saito, Ryozo Kuwano, Masayoshi Tachibana

Affiliation

¹ Division of Reconstructive Surgery for Oral and Maxillofacial Region, Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8514, Japan. [email protected]

PMID: 16525666

Abstract

Angiogenesis is induced by various angiogenic factors including vascular endothelial growth factors (VEGFs), such as VEGF-A, -B, -C and -D, and is involved in tumor progression and metastasis. In an effort to define the expression pattern of VEGFs in oral squamous cell carcinoma (OSCC) and its correlation with clinicopathological factors, we determined the expression levels of VEGFs in OSCC cell lines (HSC-2, HSC-3, HSC-4 and OSC-19) by quantitative RT-PCR and examined their relationship with regional lymph node (LN) and distant metastasis, intratumoral microvessel density (MVD) in tumor transplanted nude mice. We found that HSC-2 and OSC-19 expressed significantly higher levels of VEGF-A and VEGF-C, and caused frequent regional LN metastasis and higher MVD than did the other cell lines. Since VEGF-C is a lymphangiogenic factor, these results suggest that expression of VEGF-C is a useful predictor for LN metastasis.

MeSH terms

Analysis of Variance
Animals
Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / metabolism
Carcinoma, Squamous Cell / pathology*
Cell Line, Tumor
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Lymphatic Metastasis
Mice
Mice, Inbred BALB C
Mice, Nude
Mouth Neoplasms / genetics
Mouth Neoplasms / metabolism
Mouth Neoplasms / pathology*
Neoplasm Transplantation
Neovascularization, Pathologic
RNA, Messenger / genetics
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Transplantation, Heterologous
Vascular Endothelial Growth Factor A / analysis
Vascular Endothelial Growth Factor A / genetics*
Vascular Endothelial Growth Factor B / analysis
Vascular Endothelial Growth Factor B / genetics
Vascular Endothelial Growth Factor C / analysis
Vascular Endothelial Growth Factor C / genetics*
Vascular Endothelial Growth Factor D / analysis
Vascular Endothelial Growth Factor D / genetics

Substances

RNA, Messenger
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor B
Vascular Endothelial Growth Factor C
Vascular Endothelial Growth Factor D