Abstract
The genomic DNA of Escherichia coli, which contains the unmethylated CpG motif, was used to evaluate the immunostimulating effect of bacterial DNA on innate immune responses in the bivalve mussel Hyriopsis cumingii Lea. The results showed that the E. coli DNA had no significant effect on the production of superoxide anion (O(2-)) or acid phosphatase (AP) by haemocytes in vitro. However, the bactericidal activity of the haemocytes was significantly increased when the cells were incubated with 50 or 100mug/ml bacterial DNA for 12 and 24h. Antibacterial activity, lysozyme activity, and prophenoloxidase (proPO) production of haemolymph were also increased, when the bivalve molluscs were injected with 50 or 100mug/ml of bacterial DNA for 12 and 24h. These activities returned to the control level after 48h. This work showed the bacterial DNA with unmethylated CpG motif could activate some parameters of the immune system of bivalve molluscs in vivo and in vitro.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Acid Phosphatase / analysis
-
Adjuvants, Immunologic / pharmacology
-
Aeromonas hydrophila / physiology
-
Animals
-
Catechol Oxidase / analysis
-
Catechol Oxidase / metabolism
-
DNA, Bacterial / classification
-
DNA, Bacterial / pharmacology*
-
Enzyme Induction / drug effects
-
Enzyme Precursors / analysis
-
Enzyme Precursors / metabolism
-
Escherichia coli / genetics
-
Escherichia coli / immunology
-
Hemocytes / drug effects*
-
Hemocytes / immunology
-
Hemolymph / enzymology
-
Immunity, Innate / drug effects*
-
Lipopolysaccharides / analysis
-
Lipopolysaccharides / pharmacology
-
Muramidase / analysis
-
Muramidase / metabolism
-
Superoxides / analysis
-
Tetrazolium Salts / metabolism
-
Thiazoles / metabolism
-
Time Factors
-
Unionidae / enzymology
-
Unionidae / immunology*
Substances
-
Adjuvants, Immunologic
-
DNA, Bacterial
-
Enzyme Precursors
-
Lipopolysaccharides
-
Tetrazolium Salts
-
Thiazoles
-
Superoxides
-
pro-phenoloxidase
-
Catechol Oxidase
-
Acid Phosphatase
-
Muramidase
-
thiazolyl blue