Abstract
We characterized human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins (Env) isolated from two HIV-1-infected CCR5delta32 homozygotes. Envs from both subjects used CCR5 and CXCR4 for entry into transfected cells. Most R5X4 Envs were lymphocyte-tropic and used CXCR4 exclusively for entry into peripheral blood mononuclear cells (PBMC), but a subset was dually lymphocyte- and macrophage-tropic and used either CCR5 or CXCR4 for entry into PBMC and monocyte-derived macrophages. The persistence of CCR5-using HIV-1 in two CCR5delta32 homozygotes suggests the conserved CCR5 binding domain of Env is highly stable and provides new mechanistic insights important for HIV-1 transmission and persistence.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles*
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Amino Acid Sequence
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Base Sequence
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Cell Line
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Cells, Cultured
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Clone Cells
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Gene Products, env / chemistry
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Gene Products, env / genetics
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Gene Products, env / metabolism*
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Genes, Reporter
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HIV-1 / physiology*
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Homozygote
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Humans
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Leukocytes, Mononuclear / metabolism
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Leukocytes, Mononuclear / virology
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Luciferases / metabolism
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Male
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Molecular Sequence Data
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Receptors, CCR5 / genetics*
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Receptors, CCR5 / metabolism*
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Receptors, CXCR4 / genetics
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Receptors, CXCR4 / metabolism
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Sequence Homology, Amino Acid
Substances
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Gene Products, env
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Receptors, CCR5
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Receptors, CXCR4
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Luciferases
Associated data
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GENBANK/DQ356577
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GENBANK/DQ356584