Abstract
We have developed diffraction phase microscopy as a new technique for quantitative phase imaging of biological structures. The method combines the principles of common path interferometry and single-shot phase imaging and is characterized by subnanometer path-length stability and millisecond-scale acquisition time. The potential of the technique for quantifying nanoscale motions in live cells is demonstrated by experiments on red blood cells.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Movement / physiology
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Cells, Cultured
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Equipment Design
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Equipment Failure Analysis
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Erythrocytes / cytology*
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Erythrocytes / physiology
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Humans
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Image Enhancement / instrumentation*
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Image Enhancement / methods
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Image Interpretation, Computer-Assisted / instrumentation
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Image Interpretation, Computer-Assisted / methods
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Microscopy, Phase-Contrast / instrumentation*
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Microscopy, Phase-Contrast / methods
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Refractometry / instrumentation*
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Refractometry / methods
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Reproducibility of Results
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Sensitivity and Specificity