Abstract
We have cloned a lytic enzyme, PlyPH, with a specific lytic effect on Bacillus anthracis strains. PlyPH remains active between pH 4 and 10.5, and a single dose rescued a significant percentage of mice infected intraperitoneally with an attenuated B. anthracis strain. We propose PlyPH as a novel therapeutic agent.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Anthrax
-
Bacillus anthracis / classification
-
Bacillus anthracis / metabolism*
-
Bacillus anthracis / physiology
-
Bacteriolysis*
-
Bacteriophages / enzymology*
-
Hydrogen-Ion Concentration
-
Mice
-
Substrate Specificity
-
Viral Proteins / chemistry
-
Viral Proteins / metabolism*