Abstract
The configurations of immunoglobulin, T-cell receptor beta chain and bcl-2 genes were analyzed in lympho-proliferative disorders using nonradioactive digoxigenin-labeled probes. The studies demonstrated the reliability of digoxigenin-labeled probes for the detection of single-copy genes after Southern blotting of genomic DNA: 1 microgram and sometimes even 0.2 microgram of restriction endonuclease-digested DNA could be detected either by JH, C beta or bcl-2 probes. The intensity of the signal was consistently satisfactory, and there was no background problem. Control experiments showed neither false-positive nor false-negative results caused by the use of the nonradioactive detection system.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Blotting, Southern
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DNA / genetics
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DNA Probes*
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Digoxigenin*
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Gene Rearrangement / genetics*
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Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / genetics
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Genes, Immunoglobulin / genetics
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Humans
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Immunoglobulin J-Chains / genetics
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Lymphoproliferative Disorders / diagnosis
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Lymphoproliferative Disorders / genetics
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Lymphoproliferative Disorders / immunology
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins c-bcl-2
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Receptors, Antigen, T-Cell, alpha-beta / genetics
Substances
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DNA Probes
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Immunoglobulin J-Chains
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-bcl-2
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Receptors, Antigen, T-Cell, alpha-beta
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DNA
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Digoxigenin