Abstract
The effect of the neurotrophic factor nerve growth factor (NGF) on the expression of the beta-amyloid gene has been studied in the clonal nerve cell line PC12. The neuronal differentiation of PC12 cells in the presence of NGF was accompanied by a shift in the ratio of beta-amyloid precursor protein (APP) transcripts. In particular there was reduced expression of the transcript coding for the longest precursor form (APP770) and a concomitant increase in the shortest (APP695) transcript following NGF treatment.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amyloid beta-Protein Precursor / genetics*
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Animals
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Base Sequence
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Cell Differentiation / drug effects*
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Molecular Sequence Data
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Nerve Growth Factors / pharmacology*
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Neurons / cytology*
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Neurons / drug effects
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Neurons / physiology
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Oligodeoxyribonucleotides
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PC12 Cells
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RNA Splicing / drug effects*
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RNA, Messenger / drug effects
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RNA, Messenger / genetics*
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Transcription, Genetic
Substances
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Amyloid beta-Protein Precursor
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Nerve Growth Factors
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Oligodeoxyribonucleotides
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RNA, Messenger