Comparative study between the Light Cycler and the PCR-restriction fragment length polymorphism in detecting factor V Leiden and factor II 20210G>A mutations

Layla Mammo; Atia Sheereen; Tanya Saour; Jalal Saour

doi:10.1016/j.clinbiochem.2006.01.019

Comparative study between the Light Cycler and the PCR-restriction fragment length polymorphism in detecting factor V Leiden and factor II 20210G>A mutations

Clin Biochem. 2006 Jul;39(7):767-9. doi: 10.1016/j.clinbiochem.2006.01.019. Epub 2006 Mar 23.

Authors

Layla Mammo¹, Atia Sheereen, Tanya Saour, Jalal Saour

Affiliation

¹ Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Center, PO Box 3354, Riyadh 11211, Saudi Arabia. [email protected]

PMID: 16563366
DOI: 10.1016/j.clinbiochem.2006.01.019

Abstract

Objectives: To test reproducibility, speed and cost of testing for factor V Leiden and FII 20210G>A in our practice.

Design and methods: We compared conformity, reproducibility, speed and cost using the Light Cycler (LC) and PCR-RFLP.

Results: There was 100% conformity and reproducibility. LC was faster but 23% more expensive per sample. When equipment depreciation and patient expenses are added, LC testing becomes cheaper.

Conclusion: In our practice, LC provides fast, reproducible and cost-effective results.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

DNA Mutational Analysis / economics
DNA Mutational Analysis / methods*
Factor V / genetics*
Humans
Polymerase Chain Reaction / economics
Polymerase Chain Reaction / methods*
Polymorphism, Restriction Fragment Length
Prothrombin / genetics*
Reproducibility of Results

Substances

factor V Leiden
Factor V
Prothrombin