Abstract
The transcription factor GATA-1 is expressed in a subset of hemopoietic cells, where it mediates the cell-type specific expression of several genes. We have cloned the mouse and human GATA-1 genes. A region upstream to the first exon, and highly conserved between mouse and man, acts as an erythroid specific enhancer in transient assays, if linked to the GATA-1 or to the SV40 promoter. The activity of the enhancer is almost completely dependent on the integrity of a dimeric GATA-1 binding site.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Base Sequence
-
Binding Sites / genetics
-
Cell Line
-
Chloramphenicol O-Acetyltransferase / genetics
-
Cloning, Molecular
-
DNA-Binding Proteins / genetics*
-
DNA-Binding Proteins / metabolism
-
Enhancer Elements, Genetic / genetics*
-
Enhancer Elements, Genetic / physiology
-
Erythrocytes / metabolism*
-
Erythroid-Specific DNA-Binding Factors
-
Escherichia coli / metabolism
-
GATA1 Transcription Factor
-
Gene Expression Regulation / genetics*
-
Humans
-
Mice
-
Molecular Sequence Data
-
Promoter Regions, Genetic / genetics
-
Recombinant Fusion Proteins / biosynthesis
-
Sequence Homology, Nucleic Acid
-
Simian virus 40 / genetics
-
Transcription Factors / genetics*
-
Transcription Factors / metabolism
Substances
-
DNA-Binding Proteins
-
Erythroid-Specific DNA-Binding Factors
-
GATA1 Transcription Factor
-
GATA1 protein, human
-
Gata1 protein, mouse
-
Recombinant Fusion Proteins
-
Transcription Factors
-
Chloramphenicol O-Acetyltransferase
Associated data
-
GENBANK/S54217
-
GENBANK/S54227
-
GENBANK/S54228
-
GENBANK/S54230
-
GENBANK/S58173
-
GENBANK/S58187
-
GENBANK/S58189
-
GENBANK/S58191
-
GENBANK/X56854
-
GENBANK/X60824