Antibody production and in vitro behavior of CD27-defined B-cell subsets: persistent hepatitis C virus infection changes the rules

J Virol. 2006 Apr;80(8):3923-34. doi: 10.1128/JVI.80.8.3923-3934.2006.

Abstract

There is growing interest in the tendency of B cells to change their functional program in response to overwhelming antigen loading, perhaps by regulating specific parameters, such as efficiency of activation, proliferation rate, differentiation to antibody-secreting cells (ASC), and rate of cell death in culture. We show that individuals persistently infected with hepatitis C virus (HCV) carry high levels of circulating immunoglobulin G (IgG) and IgG-secreting cells (IgG-ASC). Thus, generalized polyclonal activation of B-cell functions may be supposed. While IgGs include virus-related and unrelated antibodies, IgG-ASC do not include HCV-specific plasma cells. Despite signs of widespread activation, B cells do not accumulate and memory B cells seem to be reduced in the blood of HCV-infected individuals. This apparent discrepancy may reflect the unconventional activation kinetics and functional responsiveness of the CD27+ B-cell subset in vitro. Following stimulation with T-cell-derived signals in the absence of B-cell receptor (BCR) engagement, CD27+ B cells do not expand but rapidly differentiate to secrete Ig and then undergo apoptosis. We propose that their enhanced sensitivity to BCR-independent noncognate T-cell help maintains a constant level of nonspecific serum antibodies and ASC and serves as a backup mechanism of feedback inhibition to prevent exaggerated B-cell responses that could be the cause of significant immunopathology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amino Acid Sequence
  • Apoptosis
  • B-Lymphocyte Subsets / immunology*
  • Female
  • Hepatitis C Antibodies / blood*
  • Hepatitis C, Chronic / immunology*
  • Humans
  • Lymphocyte Activation
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • T-Lymphocytes / physiology
  • Tumor Necrosis Factor Receptor Superfamily, Member 7 / physiology*

Substances

  • Hepatitis C Antibodies
  • Tumor Necrosis Factor Receptor Superfamily, Member 7