[Isolation of BADH gene promoter from Suaeda liaotungensis and its sequence analysis]

Sheng Wu Gong Cheng Xue Bao. 2006 Jan;22(1):77-81.
[Article in Chinese]

Abstract

In this study, the 5' -flanking proximal region of stress-induced gene encoding betaine aldehyde dehydrogenase was isolated by Adaptor-PCR and TAIL-PCR from halophyte Suaeda liaotungensis. 1993 bp sequence was obtained by sequencing. The transcription start site, which localized at 62 bases upstream of the start ATG, was predicted using TSSP-TCM program. The functional elements were analysed by PLACE programm. The SlBADH gene promoter contains the basic elements: TATA-box, CAAT-box, and stress-induced elements: salt responsed element, cold, dehydration, ABA and frozen responsed elements, WUN responsed elements and HSE. Obtaining the promoter of betaine aldehyde dehydrogenase gene from Suaeda liaotungensis provides a foundation for analyzing the stress-induced promoter elements, studying the relationship between structure and founction of the promoter, and investigating the molecular mechanism of BADH gene regulation.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Betaine-Aldehyde Dehydrogenase / genetics*
  • Chenopodiaceae / enzymology*
  • Chenopodiaceae / genetics
  • Cold Temperature
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Plant
  • Genes, Plant
  • Molecular Sequence Data
  • Plant Proteins / genetics
  • Promoter Regions, Genetic*
  • Salts
  • Sequence Analysis, DNA*
  • Transcription Initiation Site

Substances

  • Plant Proteins
  • Salts
  • Betaine-Aldehyde Dehydrogenase