Abstract
Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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ATP-Binding Cassette Transporters / genetics
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ATP-Binding Cassette Transporters / metabolism
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Arabidopsis / cytology
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Arabidopsis / growth & development
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Arabidopsis / metabolism*
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Arabidopsis / physiology
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Biological Transport
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Cell Membrane / metabolism
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Cells, Cultured
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Gravitropism
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HeLa Cells
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Humans
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Indoleacetic Acids / metabolism*
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Kinetics
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Membrane Transport Proteins / genetics
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Membrane Transport Proteins / metabolism*
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Mutation
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Naphthaleneacetic Acids / metabolism
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Nicotiana
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Phthalimides / pharmacology
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Plant Roots / physiology
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Saccharomyces cerevisiae / genetics
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Transfection
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Transformation, Genetic
Substances
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ATP-Binding Cassette Transporters
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ATPGP1 protein, Arabidopsis
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Arabidopsis Proteins
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Indoleacetic Acids
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Membrane Transport Proteins
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Naphthaleneacetic Acids
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PIN1 protein, Arabidopsis
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PIN2 protein, Arabidopsis
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PIN4 protein, Arabidopsis
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PIN7 protein, Arabidopsis
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Phthalimides
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alpha-naphthylphthalamic acid
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1-naphthaleneacetic acid