[Production and identification of monoclonal antibody against N terminal of AIB1]

Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 Mar;37(2):278-80, 297.
[Article in Chinese]

Abstract

Objective: To make and identify the monoclonal antibody against AIB1-N.

Methods: BALB/c mice were immunized with purified GST-AIB1-N protein, McAb against AIB1-N was produced by hybridoma technique. ELISA and Western-blot were used to identify the immunoglobin subtype and specificity. Results A hybridoma cell was successfully produced to secrete the McAb against AIB1-N, which was identified to belong in IgG1 subtype. By western-blot, the McAb against AIB1 displayed strongly specificity and high affinity.

Conclusion: The McAb against AIB1 protein may be a useful tool for studying the biological properties of AIB1 expression and the clinical laboratory detection.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / analysis
  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Neoplasm / biosynthesis
  • Antibodies, Neoplasm / immunology
  • Breast Neoplasms / immunology*
  • Female
  • Hybridomas
  • Mice
  • Mice, Inbred BALB C
  • Nuclear Receptor Coactivator 3
  • Transcription Factors / immunology*

Substances

  • Antibodies, Monoclonal
  • Antibodies, Neoplasm
  • Transcription Factors
  • Nuclear Receptor Coactivator 3