Abstract
Four monkeys were exposed to a retroviral vector and replication-competent murine amphotropic retrovirus in a bone marrow transplantation/gene transfer protocol (Kantoff et al., 1987). We have studied these animals 2 and 3 years post-transplantation and did not detect replicating virus in serum, peripheral blood mononuclear cells, or bone marrow cells. Amphotropic envelope sequences could not be detected in blood or bone marrow cells by Southern blotting or the polymerase chain reaction. Antibodies directed against the p30 and gp70 viral antigens were detected by Western blot and immunoprecipitation. The animals remain alive and well. Our findings suggest that primates can clear murine amphotropic retroviruses even when exposure occurs during a time of severe immunosuppression.
MeSH terms
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Adenosine Deaminase / genetics
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Animals
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Antibodies, Viral / blood
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Base Sequence
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Blood / microbiology
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Blotting, Southern
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Blotting, Western
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Bone Marrow / microbiology
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Bone Marrow Transplantation
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DNA, Viral / analysis
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Genes, env
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Genetic Engineering*
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Genetic Vectors*
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Immunocompromised Host
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Leukemia Virus, Murine / genetics
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Leukemia Virus, Murine / immunology
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Leukemia Virus, Murine / isolation & purification*
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Leukemia Virus, Murine / physiology
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Leukocytes, Mononuclear / microbiology
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Macaca fascicularis / blood
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Macaca fascicularis / immunology
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Macaca fascicularis / microbiology*
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Molecular Sequence Data
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Polymerase Chain Reaction
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Recombinant Proteins / genetics
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Retrospective Studies
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Retroviridae* / genetics
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Viremia
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Virus Replication
Substances
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Antibodies, Viral
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DNA, Viral
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Recombinant Proteins
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Adenosine Deaminase