Comparison of cytokine plasma levels in human African trypanosomiasis

Trop Med Int Health. 2006 May;11(5):647-53. doi: 10.1111/j.1365-3156.2006.01612.x.

Abstract

Background: Immunological studies suggest that human African trypanosomiasis (HAT) is associated with inflammatory responses. A better understanding of the complex cytokine interactions regulating HAT infections is essential to elucidate the mechanisms of generalized immunosuppression.

Method: We determined levels of interleukin (IL)-2, IL-4, IL-6, IL-10, tumour necrosis factor (TNF)-alpha and interferon (IFN)-gamma protein levels in plasma samples from three groups of individuals from the Democratic Republic of Congo: (i) HAT cases; (ii) seropositive individuals for whom parasite detection was negative and (ii) controls.

Results: Plasma levels of six cytokines were significantly higher in HAT cases than in both controls (P<0.003) and seropositive individuals (P<0.016). IL-2 and IL-10 concentrations were significantly lower (P<0.02) in the seropositive group than in the control one.

Conclusion: Human African trypanosomiasis leads to the development of strong cytokine responses, indicating the potential involvement of IL-2 and IL-10 in the phenomenon of seropositivity without parasitological confirmation. This strongly suggests the involvement of immunity in this particular aspect of HAT epidemiology.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cytokines / blood*
  • Cytokines / immunology
  • Democratic Republic of the Congo / epidemiology
  • Humans
  • Interferon-gamma / blood
  • Interleukin-10 / blood
  • Interleukin-2 / blood
  • Interleukin-4 / blood
  • Interleukin-6 / blood
  • Linear Models
  • Loiasis / blood
  • Loiasis / epidemiology
  • Malaria, Falciparum / blood
  • Malaria, Falciparum / epidemiology
  • Trypanosoma brucei gambiense / immunology
  • Trypanosomiasis, African / blood*
  • Trypanosomiasis, African / epidemiology
  • Trypanosomiasis, African / immunology
  • Tumor Necrosis Factor-alpha / analysis

Substances

  • Cytokines
  • Interleukin-2
  • Interleukin-6
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • Interleukin-4
  • Interferon-gamma