Knockdown of annexin 2 decreases migration of human glioma cells in vitro

Neuropathol Appl Neurobiol. 2006 Jun;32(3):271-7. doi: 10.1111/j.1365-2990.2006.00720.x.

Abstract

Diffuse invasion of brain tissue is a major reason for the poor prognosis of patients with glioblastoma. Annexin 2, a member of the large annexin family of Ca2+ and membrane-binding proteins, is expressed at high protein levels in human gliomas and has been proposed as a marker of glioma malignancy, while its functional role in these tumours is unknown so far. The ability of annexin 2 to interact with the actin cytoskeleton, as well as its potential to bind invasion-associated proteases, suggests that it could participate in invasion-associated processes in human gliomas. Therefore, we analysed here functional consequences of RNA interference-mediated silencing of annexin 2 in U87MG and U373MG human glioma cell lines. While no impact of annexin 2 downregulation on proliferation and adhesion was observed, our analyses revealed that migration of U87MG and U373MG cells was significantly inhibited following annexin 2 depletion. This effect was not related to a compensatory increase of the related annexins 1 or 6. Our findings identify annexin 2 as a potential candidate involved in glioma invasion and support the potential of RNA interference as powerful tool in the decryption of glioma invasion mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Annexin A2 / metabolism*
  • Blotting, Western
  • Brain Neoplasms / metabolism
  • Brain Neoplasms / pathology*
  • Cell Adhesion
  • Cell Line, Tumor
  • Cell Movement / physiology*
  • Down-Regulation
  • Glioma / metabolism
  • Glioma / pathology*
  • Humans
  • Immunohistochemistry
  • Neoplasm Invasiveness*
  • RNA, Small Interfering
  • Transfection

Substances

  • ANXA2 protein, human
  • Annexin A2
  • RNA, Small Interfering