Abstract
Background:
To study a new kind of adjuvant: transcutaneous immunization adjuvant.
Methods:
The full length gene of Heat-labile enterotoxin (LT) was amplified from E. coli H10407. The B subunit protein LTB and the nontoxic A subunit protein LTKA were expressed by genetic engineering manipulation. After purification, they were identified with SDS-PAGE, GM1-ELISA and so on.
Results:
The LTB protein still persisted its biologic activity that conjugated specifically with GM1 ganglioside, and the LTK63 protein lost its toxin activity.
Conclusion:
The results showed that LTB and LTK63 may be used as promising transcutaneous immunization adjuvant.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / genetics
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Adjuvants, Immunologic / isolation & purification
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Adjuvants, Immunologic / metabolism*
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Animals
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Bacterial Toxins / genetics
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Bacterial Toxins / immunology
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Bacterial Toxins / metabolism*
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Blotting, Western
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CHO Cells
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Cloning, Molecular
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Cricetinae
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Cricetulus
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Electrophoresis, Polyacrylamide Gel
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Enterotoxins / genetics
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Enterotoxins / immunology
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Enterotoxins / metabolism*
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / immunology
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Escherichia coli Proteins / metabolism*
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Gene Expression
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Genetic Engineering
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Plasmids / genetics
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / immunology
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Recombinant Fusion Proteins / metabolism*
Substances
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Adjuvants, Immunologic
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Bacterial Toxins
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Enterotoxins
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Escherichia coli Proteins
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Recombinant Fusion Proteins
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heat-labile enterotoxin, E coli